Antigen

Antigen Class

TFs and others

Antigen

Rai1

Cell type

Cell type Class

Neural

Cell type

Cortex

NA

NA

Sample

source_name

Cortex-Rai-ChIP

strain

B6/129

generation

F1

tissue

Cortex

chip antibody

anti-FLAG M2 (Sigma, catalog# F1804)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Rai1-DNA complexes were isolated with anti-FLAG antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Nextera DNA Library Prep Kit).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

36695403

Reads aligned (%)

96.5

Duplicates removed (%)

11.0

Number of peaks

13166 (qval < 1E-05)

mm9

Number of total reads

36695403

Reads aligned (%)

96.4

Duplicates removed (%)

11.8

Number of peaks

13100 (qval < 1E-05)